HopCellTM human iPSC/ESC-Derived cortical neurons are populations representative of the six cortical layers with both excitatory glutaminergic neurons (about 70-80%) and GABAergic interneurons (about 20-30%). The neurons are electrically active and can form functional synapses and neuronal networks in vitro, starting from day 7-14 after inducing differentiation. Due to its purity and stability, this culture can be maintained to more than 3 years in vitro.
Cortical neurons (Day 14 of differentiation)
VGAT (Green) VGLUT (Red) DAPI(Blue) (Day 56)
MAP2 (Green) Synapsin (Red) DAPI(Blue) (Day 49)
PSD95(Green) MAP2 (Red) DAPI(Blue) (Day 63)
Electrophysiology activities (Day 14 of differentiation)